KD-Validated CDC123 Mouse mAb (20 μl)

KD-Validated CDC123 Mouse mAb (20 μl)

Cat. #: 63454
Availability: In Stock
$149.00
-+
Reactivity:   Human    
Applications:   WB, FC    
Host Species:   Mouse    
Isotype:   IgG2b    
Clonality:   Monoclonal antibody    
Gene Name:   cell division cycle 123
Gene Symbol:   CDC123
Synonyms:   D123; C10orf7
Gene ID:   8872
UniProt ID:   O75794
Clone ID:   24GB5705
Immunogen:   Recombinant protein of human CDC123
Dilution:   WB 1:2,500-1:5,000; FC 1:2,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

Enables ATP binding activity and magnesium ion binding activity. Involved in eukaryotic translation initiation factor 2 complex assembly. Located in cytoplasm.




Images

CDC123 antibodyWestern blotting analysis using anti-cell division cycle 123 antibody (Cat#63454). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle 123 antibody (Cat#63454, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-cell division cycle 123 antibody (Cat#63454). Cell division cycle 123 expression in wild type (WT) and cell division cycle 123 (CDC123) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cell division cycle 123 antibody (Cat#63454, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cell division cycle 123 expression in HepG2 cells using anti-cell division cycle 123 antibody (Cat#63454, 1:2,000). Green, isotype control; red, cell division cycle 123.



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