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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cell division cycle 45 |
| Gene Symbol: | CDC45 |
| Synonyms: | CDC45L; CDC45L2; Cell division control protein 45 homolog; PORC-PI-1 |
| Gene ID: | 8318 |
| UniProt ID: | O75419 |
| Clone ID: | 25GB2945 |
| Immunogen: | A synthesized peptide derived from human CDC45 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-cell division cycle 45 antibody (Cat#61590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle 45 antibody (Cat#61590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-cell division cycle 45 antibody (Cat#61590). Cell division cycle 45 expression in wild type (WT) and cell division cycle 45 (CDC45) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cell division cycle 45 antibody (Cat#61590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Cell division cycle 45 expression in HepG2 cells using anti-Cell division cycle 45 antibody (Cat#61590, 1:2,000). Green, isotype control; red, Cell division cycle 45. |
 | Immunocytochemical staining of HepG2 cells with anti-Cell division cycle 45 antibody (Cat#61590, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle 45 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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