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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cyclin dependent kinase inhibitor 2A |
| Gene Symbol: | CDKN2A |
| Synonyms: | ARF; MLM; P14; P16; P19; CAI2; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A |
| Gene ID: | 1029 |
| UniProt ID: | P42771 |
| Clone ID: | 25GB3420 |
| Immunogen: | A synthesized peptide derived from human p16 INK |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene CDKN2A generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene; this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants. This ARF product functions as a stabilizer of the tumor suppressor protein p53 as it can interact with, and sequester, the E3 ubiquitin-protein ligase MDM2, a protein responsible for the degradation of p53. In spite of the structural and functional differences, the CDK inhibitor isoforms and the ARF product encoded by this gene, through the regulatory roles of CDK4 and p53 in cell cycle G1 progression, share a common functionality in cell cycle G1 control. This gene is frequently mutated or deleted in a wide variety of tumors, and is known to be an important tumor suppressor gene.
Images
 | Western blotting analysis using anti-cyclin dependent kinase inhibitor 2A antibody (Cat#61716). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin dependent kinase inhibitor 2A antibody (Cat#61716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-cyclin dependent kinase inhibitor 2A antibody (Cat#61716). Cyclin dependent kinase inhibitor 2A expression in wild-type (WT) and cyclin dependent kinase inhibitor 2A (CDKN2A) knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin dependent kinase inhibitor 2A antibody (Cat#61716, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Cyclin dependent kinase inhibitor 2A expression in HeLa cells using anti-Cyclin dependent kinase inhibitor 2A antibody (Cat#61716, 1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 2A. |
 | Immunocytochemical staining of Hela cells with Cyclin dependent kinase inhibitor 2A antibody (Cat#61716, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Scale bar, 20 μm. |
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