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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | cilia and flagella associated protein 298 |
| Gene Symbol: | CFAP298 |
| Synonyms: | Kur; FBB18; CILD26; DNAAF16; C21orf48; C21orf59 |
| Gene ID: | 56683 |
| UniProt ID: | P57076 |
| Clone ID: | 24GB7915 |
| Immunogen: | Recombinant protein of human C21orf59 |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein that plays a critical role in dynein arm assembly and motile cilia function. Mutations in this gene result in primary ciliary dyskinesia. Naturally occuring readthrough transcription occurs from this locus to the downstream t-complex 10 like (TCP10L) gene.
Images
 | Western blotting analysis using anti-CFAP298 antibody (Cat#63796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CFAP298 antibody (Cat#63796, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-CFAP298 antibody (Cat#63796). CFAP298 expression in wild type (WT) and CFAP298 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CFAP298 antibody (Cat#63796, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of CFAP298 expression in HepG2 cells using anti-CFAP298 antibody (Cat#63796, 1:1,000). Green, isotype control; red, CFAP298. |
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