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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | coiled-coil-helix-coiled-coil-helix domain containing 3 |
| Gene Symbol: | CHCHD3 |
| Synonyms: | Mic19; MINOS3; MICOS19; PPP1R22 |
| Gene ID: | 54927 |
| UniProt ID: | Q9NX63 |
| Clone ID: | 24GB6560 |
| Immunogen: | Recombinant protein of human CHCHD3 |
| Dilution: | WB 1:2,500-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-CHCHD3 antibody (Cat#63568). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHCHD3 antibody (Cat#63568, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CHCHD3 antibody (Cat#63568). CHCHD3 expression in wild type (WT) and CHCHD3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CHCHD3 antibody (Cat#63568, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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