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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | creatine kinase B |
| Gene Symbol: | CKB |
| Synonyms: | BCK; B-CK; CKBB; CPK-B; HEL-211; HEL-S-29 |
| Gene ID: | 1152 |
| UniProt ID: | P12277 |
| Clone ID: | 24GB4355 |
| Immunogen: | A synthesized peptide derived from human Creatine kinase B type |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CKB encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
Images
 | Western blotting analysis using anti-creatine kinase B antibody (Cat#63172). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-creatine kinase B antibody (Cat#63172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-creatine kinase B antibody (Cat#63172). Creatine kinase B expression in wild-type (WT) and creatine kinase B (CKB) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-creatine kinase B antibody (Cat#63172, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Creatine kinase B expression in HeLa cells using anti-Creatine kinase B antibody (Cat#63172, 1:2,000). Green, isotype control; red, Creatine kinase B. |
 | Immunocytochemical staining of Hela cells with creatine kinase B antibody (Cat#63172, 1:1,000). Nuclei were stained blue with DAPI; Creatine kinase B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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