KD-Validated CMPK1 Mouse mAb (20 μl)

KD-Validated CMPK1 Mouse mAb (20 μl)

Cat. #: 63806
Availability: In Stock
$149.00
-+
Reactivity:   Human    
Applications:   WB, FC    
Host Species:   Mouse    
Isotype:   IgG1    
Clonality:   Monoclonal antibody    
Gene Name:   cytidine/uridine monophosphate kinase 1
Gene Symbol:   CMPK1
Synonyms:   CK; CMK; UMK; CMPK; UMPK; UMP-CMPK
Gene ID:   51727
UniProt ID:   P30085
Clone ID:   24GB7965
Immunogen:   Recombinant protein of human CMPK1
Dilution:   WB 1:500-1:2,500; FC 1:1,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants.




Images

CMPK1 antibodyWestern blotting analysis using anti-CMPK1 antibody (Cat#63806). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CMPK1 antibody (Cat#63806, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-CMPK1 antibody (Cat#63806). CMPK1 expression in wild type (WT) and CMPK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CMPK1 antibody (Cat#63806, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CMPK1 expression in HepG2 cells using anti-CMPK1 antibody (Cat#63806, 1:1,000). Green, isotype control; red, CMPK1.



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