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| Reactivity: | Human, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | CASP2 and RIPK1 domain containing adaptor with death domain |
| Gene Symbol: | CRADD |
| Synonyms: | MRT34; RAIDD |
| Gene ID: | 8738 |
| UniProt ID: | P78560 |
| Clone ID: | 25GB3380 |
| Immunogen: | A synthesized peptide derived from human RAIDD |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein containing a death domain (DD) motif. This protein recruits caspase 2/ICH1 to the cell death signal transduction complex, which includes tumor necrosis factor receptor 1 (TNFR1A) and RIPK1/RIP kinase, and acts in promoting apoptosis. A mutation in this gene was associated with cognitive disability. A related pseudogene is found on chromosome 3. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-CRADD antibody (Cat#61701). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRADD antibody (Cat#61701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-CRADD antibody (Cat#61701). CRADD expression in wild type (WT) and CRADD knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CRADD antibody (Cat#61701, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of CRADD expression in HepG2 cells using anti-CRADD antibody (Cat#61701, 1:2,000). Green, isotype control; red, CRADD. |
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