Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | CREB regulated transcription coactivator 3 |
| Gene Symbol: | CRTC3 |
| Synonyms: | TORC3; TORC-3 |
| Gene ID: | 64784 |
| UniProt ID: | Q6UUV7 |
| Clone ID: | 24GB5935 |
| Immunogen: | A synthesized peptide derived from CRTC3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms.
Images
 | Western blotting analysis using anti-CRTC3 antibody (Cat#61131). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CRTC3 antibody (Cat#61131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-CRTC3 antibody (Cat#61131). CRTC3 expression in wild type (WT) and CRTC3 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CRTC3 antibody (Cat#61131, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of CRTC3 expression in HeLa cells using CRTC3 antibody (Cat#61131, 1:2,000). Green, isotype control; red, CRTC3. |
 | Immunocytochemical staining of Hela cells with CRTC3 antibody (Cat#61131, 1:1,000). Nuclei were stained blue with DAPI; CRTC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
You may also be interested in:
