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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | casein kinase 1 epsilon |
| Gene Symbol: | CSNK1E |
| Synonyms: | CKIe; HCKIE; CKIepsilon |
| Gene ID: | 1454 |
| UniProt ID: | P49674 |
| Clone ID: | 25GB490 |
| Immunogen: | Recombinant protein of human CSNK1E |
| Dilution: | WB 1:1,000-1:2,500; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CSNK1E encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.
Images
 | Western blotting analysis using anti-casein kinase 1 epsilon antibody (Cat#61250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-casein kinase 1 epsilon antibody (Cat#61250). Casein kinase 1 epsilon expression in wild type (WT) and casein kinase 1 epsilon (CSNK1E) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-casein kinase 1 epsilon antibody (Cat#61250, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Casein kinase 1 epsilon expression in HT-1080 cells using anti-Casein kinase 1 epsilon antibody (Cat# 61250, 1:2,000). Green, isotype control; red, Casein kinase 1 epsilon. |
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