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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | DNA damage inducible transcript 3 |
| Gene Symbol: | DDIT3 |
| Synonyms: | CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; AltDDIT3; C/EBPzeta |
| Gene ID: | 1649 |
| UniProt ID: | P35638 |
| Clone ID: | 25GB2955 |
| Immunogen: | A synthesized peptide derived from human DDIT3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene DDIT3 encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.
Images
 | Western blotting analysis using anti-DNA damage inducible transcript 3 antibody (Cat#61591). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA damage inducible transcript 3 antibody (Cat#61591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-DNA damage inducible transcript 3 antibody (Cat#61591). DNA damage inducible transcript 3 expression in wild-type (WT) and DNA damage inducible transcript 3 (DDIT3) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA damage inducible transcript 3 antibody (Cat#61591, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of DNA damage inducible transcript 3 expression in HepG2 cells using anti-DNA damage inducible transcript 3c antibody (Cat#61591, 1:2,000). Green, isotype control; red, DNA damage inducible transcript 3. |
 | Immunocytochemical staining of HepG2 cells with anti-DNA damage inducible transcript 3 antibody (Cat#61591, 1:1,000). Nuclei were stained blue with DAPI; DNA damage inducible transcript 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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