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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | DExD-box helicase 50 |
| Gene Symbol: | DDX50 |
| Synonyms: | GU2; GUB; mcdrh; RH-II/GuB |
| Gene ID: | 79009 |
| UniProt ID: | Q9BQ39 |
| Clone ID: | 24GB8345 |
| Immunogen: | Recombinant protein of human DDX50 |
| Dilution: | WB 1:500-1:2,500; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.
Images
 | Western blotting analysis using anti-DDX50 antibody (Cat#63883). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX50 antibody (Cat#63883, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-DDX50 antibody (Cat#63883). DDX50 expression in wild type (WT) and DDX50 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-DDX50 antibody (Cat#63883, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of H9c2 cells with anti-DDX50 antibody (Cat#63883, 1:1,000). Nuclei were stained blue with DAPI; DDX50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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