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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | dihydropyrimidine dehydrogenase |
| Gene Symbol: | DPYD |
| Synonyms: | DHP; DPD; DYPD; DHPDHASE |
| Gene ID: | 1806 |
| UniProt ID: | Q12882 |
| Clone ID: | 24GB5990 |
| Immunogen: | A synthesized peptide derived from human DPYD |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a pyrimidine catabolic enzyme and the initial and rate-limiting factor in the pathway of uracil and thymidine catabolism. Mutations in this gene result in dihydropyrimidine dehydrogenase deficiency, an error in pyrimidine metabolism associated with thymine-uraciluria and an increased risk of toxicity in cancer patients receiving 5-fluorouracil chemotherapy. Two transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-dihydropyrimidine dehydrogenase antibody (Cat#61151). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dihydropyrimidine dehydrogenase antibody (Cat#61151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-Dihydropyrimidine dehydrogenase antibody (Cat#61151). Dihydropyrimidine dehydrogenase expression in wild type (WT) and Dihydropyrimidine dehydrogenase shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Dihydropyrimidine dehydrogenase antibody (Cat#61151, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using Plus ECL Super Kit (Cat#MR1004). |
 | Flow cytometric analysis of dihydropyrimidine dehydrogenase expression in H9c2 cells using dihydropyrimidine dehydrogenase antibody (Cat#61151, 1:2,000). Green, isotype control; red, dihydropyrimidine dehydrogenase. |
 | Immunocytochemical staining of H9C2 cells with anti-dihydropyrimidine dehydrogenase antibody (Cat#61151, 1:1,000). Nuclei were stained blue with DAPI; Dihydropyrimidine dehydrogenase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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