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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | epithelial cell transforming 2 |
| Gene Symbol: | ECT2 |
| Synonyms: | ARHGEF31 |
| Gene ID: | 1894 |
| UniProt ID: | Q9H8V3 |
| Clone ID: | 24GB8265 |
| Immunogen: | Recombinant protein of human ECT2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein ECT2 encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-epithelial cell transforming 2 antibody (Cat#63867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epithelial cell transforming 2 antibody (Cat#63867, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-epithelial cell transforming 2 antibody (Cat#63867). Epithelial cell transforming 2 expression in wild type (WT) and epithelial cell transforming 2 (ECT2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epithelial cell transforming 2 antibody (Cat#63867, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Epithelial cell transforming 2 expression in HT-1080 cells using anti-Epithelial cell transforming 2 antibody (Cat#63867, 1:1,000). Green, isotype control; red, Epithelial cell transforming 2. |
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