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Reactivity: | Human, Mouse, Rat |
Applications: | WB |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | eukaryotic translation elongation factor 2 |
Gene Symbol: | EEF2 |
Synonyms: | EF2; EF-2; EEF-2; SCA26 |
Gene ID: | 1938 |
UniProt ID: | P13639 |
Immunogen: | A synthesized peptide derived from human EEF2 |
Dilution: | WB 1:500-1:2,500 |
Purification Method: | Affinity purified |
Concentration: | Lot dependent |
Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene EEF2 encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.
Images
![]() | Western blotting analysis using anti-EEF2 antibody (Cat#64990). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EEF2 antibody (Cat#64990, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
![]() | Western blotting analysis using anti-EEF2 antibody (Cat#64990). EEF2 expression in wild-type (WT) and EEF2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EEF2 antibody (Cat#64990, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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