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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 2B subunit gamma |
| Gene Symbol: | EIF2B3 |
| Synonyms: | VWM3; EIF-2B; EIF2Bgamma |
| Gene ID: | 8891 |
| UniProt ID: | Q9NR50 |
| Clone ID: | 24GB8150 |
| Immunogen: | Recombinant protein of human EIF2B3 |
| Dilution: | WB 1:500-1:2,500; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-EIF2B3 antibody (Cat#63844). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF2B3 antibody (Cat#63844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-EIF2B3 antibody (Cat#63844). EIF2B3 expression in wild type (WT) and EIF2B3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF2B3 antibody (Cat#63844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of EIF2B3 expression in C2C12 cells using anti-EIF2B3 antibody (Cat#63844, 1:1,000). Green, isotype control; red, EIF2B3. |
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