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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | eukaryotic translation initiation factor 3 subunit B |
| Gene Symbol: | EIF3B |
| Synonyms: | PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116 |
| Gene ID: | 8662 |
| UniProt ID: | P55884 |
| Clone ID: | 24GB4545 |
| Immunogen: | A synthesized peptide derived from human eIF3B |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables RNA binding activity. Contributes to translation initiation factor activity. Involved in several processes, including IRES-dependent viral translational initiation; translational initiation; and viral translational termination-reinitiation. Located in cytoplasmic stress granule. Part of eukaryotic translation initiation factor 3 complex.
Images
 | Western blotting analysis using anti-EIF3B antibody (Cat#63215). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3B antibody (Cat#63215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). EIF3B, eukaryotic translation initiation factor 3 subunit B. |
 | Western blotting analysis using anti-EIF3B antibody (Cat#63215). EIF3B expression in wild type (WT) and EIF3B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EIF3B antibody (Cat#63215, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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