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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | exocyst complex component 2 |
| Gene Symbol: | EXOC2 |
| Synonyms: | SEC5; Sec5p; SEC5L1; NEDFACH |
| Gene ID: | 55770 |
| UniProt ID: | Q96KP1 |
| Clone ID: | 25GB3030 |
| Immunogen: | A synthesized peptide derived from human EXOC2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a component of the exocyst complex, a multi-protein complex essential for the polarized targeting of exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and the functions of the exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. This interaction has been shown to mediate filopodia formation in fibroblasts. This protein has been shown to interact with the Ral subfamily of GTPases and thereby mediate exocytosis by tethering vesicles to the plasma membrane. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-exocyst complex component 2 antibody (Cat#61615). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-exocyst complex component 2 antibody (Cat#61615, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-exocyst complex component 2 antibody (Cat#61615). Exocyst complex component 2 expression in wild type (WT) and exocyst complex component 2 (EXOC2) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-exocyst complex component 2 antibody (Cat#61615, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Exocyst complex component 2 expression in HepG2 cells using anti-Exocyst complex component 2 antibody (Cat#61615, 1:2,000). Green, isotype control; red, Exocyst complex component 2. |
 | Immunocytochemical staining of HepG2 cells with anti-Exocyst complex component 2 antibody (Cat#61615, 1:1,000). Nuclei were stained blue with DAPI; Exocyst complex component 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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