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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | glutamine--fructose-6-phosphate transaminase 1 |
| Gene Symbol: | GFPT1 |
| Synonyms: | GFA; GFAT; GFPT; MSLG; CMS12; GFAT1; CMSTA1; GFAT 1; GFAT1m; GFPT1L |
| Gene ID: | 2673 |
| UniProt ID: | Q06210 |
| Clone ID: | 25GB2800 |
| Immunogen: | A synthesized peptide derived from human GFPT1 |
| Dilution: | WB 1:4,000-1:20,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene GFPT1 encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate.
Images
 | Western blotting analysis using anti-GFPT1 antibody (Cat#61551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GFPT1 antibody (Cat#61551, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-GFPT1 antibody (Cat#61551). GFPT1 expression in wild type (WT) and GFPT1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GFPT1 antibody (Cat#61551, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of HepG2 cells with anti-GFPT1 antibody (Cat#61551, 1:1000). Nuclei were stained blue with DAPI; GFPT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meidum. Scale bar, 20 μm. |
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