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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | geminin DNA replication inhibitor |
| Gene Symbol: | GMNN |
| Synonyms: | Gem; MGORS6 |
| Gene ID: | 51053 |
| UniProt ID: | O75496 |
| Clone ID: | 25GB3475 |
| Immunogen: | Recombinant protein of human Geminin |
| Dilution: | WB 1:4,000-1:20,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.
Images
 | Western blotting analysis using anti-GMNN antibody (Cat#61731). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GMNN antibody (Cat#61731, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-GMNN antibody (Cat#61731). GMNN expression in wild-type (WT) and GMNN knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GMNN antibody (Cat#61731, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Immunocytochemical staining of H9C2 cells with anti-GMNN antibody (Cat#61731, 1:1,000). Nuclei were stained blue with DAPI; GMNN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Low. Scale bar, 20 μm. |
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