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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | general transcription factor IIF subunit 2 |
| Gene Symbol: | GTF2F2 |
| Synonyms: | BTF4; RAP30; TF2F2; TFIIF |
| Gene ID: | 2963 |
| UniProt ID: | P13984 |
| Clone ID: | 24GB6035 |
| Immunogen: | A synthesized peptide derived from human GTF2F2 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in positive regulation of transcription by RNA polymerase II; transcription elongation by RNA polymerase II; and transcription initiation at RNA polymerase II promoter. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription factor TFIIF complex.
Images
 | Western blotting analysis using anti-GTF2F2 antibody (Cat#61163). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GTF2F2 antibody (Cat#61163, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-GTF2F2 antibody (Cat#61163). GTF2F2 expression in wild type (WT) and GTF2F2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-GTF2F2 antibody (Cat#61163, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of HepG2 cells with anti-GTF2F2 antibody (Cat#61163, 1:1,000). Nuclei were stained blue with DAPI; GTF2F2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm. |
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