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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | 3-hydroxyisobutyryl-CoA hydrolase |
| Gene Symbol: | HIBCH |
| Synonyms: | HIBYLCOAH |
| Gene ID: | 26275 |
| UniProt ID: | Q6NVY1 |
| Clone ID: | 24GB13045 |
| Immunogen: | Recombinant protein of human HIBCH |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes the enzyme responsible for hydrolysis of both HIBYL-CoA and beta-hydroxypropionyl-CoA. Mutations in this gene have been associated with 3-hyroxyisobutyryl-CoA hydrolase deficiency. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-HIBCH antibody (Cat#64808). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIBCH antibody (Cat#……, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-HIBCH antibody (Cat#64808). HIBCH expression in wild-type (WT) and HIBCH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIBCH antibody (Cat#64808, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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