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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | hypoxia inducible factor 1 subunit alpha inhibitor |
| Gene Symbol: | HIF1AN |
| Synonyms: | FIH1 |
| Gene ID: | 55662 |
| UniProt ID: | Q9NWT6 |
| Clone ID: | 23GB6385 |
| Immunogen: | A synthesized peptide derived from human HIF1AN |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables several functions, including 2-oxoglutarate-dependent dioxygenase activity; NF-kappaB binding activity; and transition metal ion binding activity. Involved in negative regulation of Notch signaling pathway and positive regulation of myoblast differentiation. Located in cytosol; nucleoplasm; and perinuclear region of cytoplasm.
Images
 | Western blotting analysis using anti-HIF1AN antibody (Cat#62181). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-HIF1AN antibody (Cat#62181). HIF1AN expression in wild type (WT) and HIF1AN shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HIF1AN antibody (Cat#62181, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of HIF1AN expression in HeLa cells using HIF1AN antibody (Cat#62181, 1:2,000). Green, isotype control; red, HIF1AN. |
 | Immunocytochemical staining of HeLa cells with anti-HIF1AN antibody (Cat#62181, 1:1,000). Nuclei were stained blue with DAPI; HIF1AN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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