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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | left-right determination factor 1 |
| Gene Symbol: | LEFTY1/LEFTY2 |
| Synonyms: | LEFTB; LEFTYB |
| Gene ID: | 10637, 7044 |
| UniProt ID: | O75610, O00292 |
| Clone ID: | 24GB6115 |
| Immunogen: | A synthesized peptide derived from human LEFTY1 + LEFTY2 |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. The protein may also play a role in endometrial bleeding. Mutations in this gene have been associated with left-right axis malformations, particularly in the heart and lungs. Some types of infertility have been associated with dysregulated expression of this gene in the endometrium. This gene is closely linked to both a related family member and a related pseudogene. This gene encodes multiple isoforms that may undergo similar proteolytic processing.
Images
 | Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-LEFTY1 + LEFTY2 antibody (Cat#61190). LEFTY1 + LEFTY2 expression in wild type (WT) and LEFTY1 + LEFTY2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of HepG2 cells with anti-LEFTY1 + LEFTY2 antibody (Cat#61190, 1:1,000). Nuclei were stained blue with DAPI; LEFTY1 + LEFTY2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm. |
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