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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | microtubule associated protein 1 light chain 3 beta |
| Gene Symbol: | MAP1LC3B |
| Synonyms: | LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3 |
| Gene ID: | 81631 |
| UniProt ID: | Q9GZQ8 |
| Clone ID: | 25GB3515 |
| Immunogen: | A synthesized peptide derived from human LC3B |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Images
 | Western blotting analysis using anti-LC3B antibody (Cat#61743). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LC3B antibody (Cat#61743, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-LC3B antibody (Cat#61743). LC3B expression in wild-type (WT) and MAP1LC3B knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-LC3B antibody (Cat#61743, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of LC3B expression in H9c2 cells using anti-LC3B antibody (Cat#61743, 1:2,000). Green, isotype control; red, LC3B. |
 | Immunocytochemical staining of H9C2 cells with anti-LC3B antibody (Cat#61743, 1:1,000). Nuclei were stained blue with DAPI; LC3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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