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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | methionyl-tRNA synthetase 1 |
| Gene Symbol: | MARS1 |
| Synonyms: | MRS; ILLD; MARS; TTD9; CMT2U; ILFS2; METRS; MTRNS; SPG70 |
| Gene ID: | 4141 |
| UniProt ID: | P56192 |
| Clone ID: | 25GB185 |
| Immunogen: | A synthesized peptide derived from human MARS |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules.
Images
 | Western blotting analysis using anti-MARS1 antibody (Cat#65212). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MARS1 antibody (Cat#65212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-MARS1 antibody (Cat#65212). MARS1 expression in wild-type (WT) and MARS1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MARS1 antibody (Cat#65212, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MARS1 expression in HT-1080 cells using anti-MARS1 antibody (Cat#65212, 1:2,000). Green, isotype control; red, MARS1. |
 | Immunocytochemical staining of HT-1080 cells with anti-MARS1 antibody (Cat#65212, 1:1,000). Nuclei were stained blue with DAPI; MARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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