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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | MIB E3 ubiquitin protein ligase 1 |
| Gene Symbol: | MIB1 |
| Synonyms: | MIB; DIP1; ZZZ6; DIP-1; LVNC7; ZZANK2 |
| Gene ID: | 57534 |
| UniProt ID: | Q86YT6 |
| Clone ID: | 25GB420 |
| Immunogen: | A synthesized peptide derived from human MIB1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
Images
 | Western blotting analysis using anti-MIB1 antibody (Cat#65263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MIB1 antibody (Cat#65263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-MIB1 antibody (Cat#65263). MIB1 expression in wild-type (WT) and MIB1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MIB1 antibody (Cat#65263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of MIB1 expression in HepG2 cells using anti-MIB1 antibody (Cat#65263, 1:2,000). Green, isotype control; red, MIB1. |
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