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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | membrane metalloendopeptidase |
| Gene Symbol: | MME |
| Synonyms: | NEP; SFE; CD10; CALLA; CMT2T; SCA43 |
| Gene ID: | 4311 |
| UniProt ID: | P08473 |
| Clone ID: | 25GB480 |
| Immunogen: | A synthesized peptide derived from human CD10 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Images
 | Western blotting analysis using anti-CD10 antibody (Cat#61234). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-CD10 antibody (Cat#61234). CD10 expression in wild type (WT) and CD10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CD10 antibody (Cat#61234, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed usingNaQ™ ECL Substrate Kit (Cat#716). |
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