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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Mov10 RNA helicase |
| Gene Symbol: | MOV10 |
| Synonyms: | gb110; fSAP113 |
| Gene ID: | 4343 |
| UniProt ID: | Q9HCE1 |
| Clone ID: | 24GB575 |
| Immunogen: | A synthesized peptide derived from human Mov10 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables 5'-3' RNA helicase activity and RNA binding activity. Involved in defense response to virus and mRNA destabilization. Located in P-body and cytosol. Implicated in hypertension.
Images
 | Western blotting analysis using anti-MOV10 antibody (Cat#62382). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MOV10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Mov10 antibody (Cat#62382). Mov10 expression in wild type (WT) and Mov10 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Mov10 antibody (Cat#62382, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MOV10 expression in Hela cells using anti-MOV10 antibody (Cat#62382, 1:2,000). Green, isotype control; red, MOV10. |
 | Immunocytochemical staining of HeLa cells with anti-MOV10 antibody (Cat#62382, 1:1,000). Nuclei were stained blue with DAPI; MOV10 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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