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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | MYD88 innate immune signal transduction adaptor |
| Gene Symbol: | MYD88 |
| Synonyms: | WM1; IMD68; MYD88D |
| Gene ID: | 4615 |
| UniProt ID: | Q99836 |
| Clone ID: | 24GB240 |
| Immunogen: | A synthesized peptide derived from human MyD88 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a cytosolic adapter protein that plays a central role in the innate and adaptive immune response. This protein functions as an essential signal transducer in the interleukin-1 and Toll-like receptor signaling pathways. These pathways regulate that activation of numerous proinflammatory genes. The encoded protein consists of an N-terminal death domain and a C-terminal Toll-interleukin1 receptor domain. Patients with defects in this gene have an increased susceptibility to pyogenic bacterial infections. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-MyD88 antibody (Cat#62307). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MyD88 antibody (Cat#62307, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-MyD88 antibody (Cat#62307). MyD88 expression in wild type (WT) and MyD88 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MyD88 antibody (Cat#62307, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of MyD88 expression in HAP-1 cells using anti-MyD88 antibody (Cat#62307, 1:2,000). Green, isotype control; red, MyD88. |
 | Immunocytochemical staining of HAP-1 cells with anti-MyD88 antibody (Cat#62307, 1:1,000). Nuclei were stained blue with DAPI; MyD88 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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