Your shopping cart is empty!
| Reactivity: | Human, Mouse |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | myosin IB |
| Gene Symbol: | MYO1B |
| Synonyms: | MMIa; myr1; MYH-1c; MMI-alpha |
| Gene ID: | 4430 |
| UniProt ID: | O43795 |
| Clone ID: | 25GB3015 |
| Immunogen: | A synthesized peptide derived from human MYO1B |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables ATP binding activity; actin filament binding activity; and microfilament motor activity. Involved in actin filament organization and post-Golgi vesicle-mediated transport. Located in several cellular components, including actin filament; endosome; and trans-Golgi network membrane.
Images
 | Western blotting analysis using anti-myosin IB antibody (Cat#61610). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-myosin IB antibody (Cat#61610, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-myosin IB antibody (Cat#61610). Myosin IB expression in wild-type (WT) and myosin IB (MYO1B) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-myosin IB antibody (Cat#61610, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Myosin IB expression in HepG2 cells using anti-Myosin IBc antibody (Cat#61610, 1:2,000). Green, isotype control; red, Myosin IB. |
 | lmmunocytochemical staining of HepG2 cells with Myosin IB antibody (Cat#61610, 1:1,000). Nuclei were stained blue with DAPl; Myosin IB was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
You may also be interested in:
