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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NADH:ubiquinone oxidoreductase subunit B1 |
| Gene Symbol: | NDUFB1 |
| Synonyms: | MNLL; CI-MNLL; CI-SGDH |
| Gene ID: | 4707 |
| UniProt ID: | O75438 |
| Clone ID: | 24GB13735 |
| Immunogen: | Recombinant protein of human NDUFB1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable NADH dehydrogenase (ubiquinone) activity. Involved in mitochondrial respiratory chain complex I assembly. Located in mitochondrion and nuclear speck. Part of mitochondrial inner membrane and respiratory chain complex I.
Images
 | Western blotting analysis using anti-NDUFB1 antibody (Cat#64915). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFB1 antibody (Cat#64915, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-NDUFB1 antibody (Cat#64915). NDUFB1 expression in wild-type (WT) and NDUFB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NDUFB1 antibody (Cat#64915, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of NDUFB1 expression in HepG2 cells using anti-NDUFB1 antibody (Cat#64915, 1:1,000). Green, isotype control; red, NDUFB1. |
 | Immunocytochemical staining of HepG2 cells with anti-NDUFB1 antibody (Cat#64915, 1:1,000). Nuclei were stained blue with DAPI; NDUFB1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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