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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | nuclear factor kappa B subunit 2 |
| Gene Symbol: | NFKB2 |
| Synonyms: | p52; p100; H2TF1; LYT10; CVID10; LYT-10; NF-kB2; p49/p100 |
| Gene ID: | 4791 |
| UniProt ID: | Q00653 |
| Clone ID: | 25GB2990 |
| Immunogen: | A synthesized peptide derived from human NFkB p100 / p52 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-nuclear factor kappa B subunit 2 antibody (Cat#61602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-nuclear factor kappa B subunit 2 antibody (Cat#61602). Nuclear factor kappa B subunit 2 expression in wild-type (WT) and nuclear factor kappa B subunit 2 (NFKB2) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Nuclear factor kappa B subunit 2 expression in HepG2 cells using anti-Nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:2,000). Green, isotype control; red, Nuclear factor kappa B subunit 2. |
 | lmmunocytochemical staining of HepG2 cells with Nuclear factor kappa B subunit 2 antibody (Cat#61602, 1:1,000). Nuclei were stained blue with DAPl; Nuclear factor kappa B subunit 2 was stained magenta with Alexa Fluor® 647. lmages were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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