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| Reactivity: | Human, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | NHERF family PDZ scaffold protein 1 |
| Gene Symbol: | NHERF1 |
| Synonyms: | EBP50; NHERF; NHE-RF; NHERF-1; NPHLOP2; SLC9A3R1 |
| Gene ID: | 9368 |
| UniProt ID: | O14745 |
| Clone ID: | 24GB1270 |
| Immunogen: | A synthesized peptide derived from human EBP50 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a sodium/hydrogen exchanger regulatory cofactor. The protein interacts with and regulates various proteins including the cystic fibrosis transmembrane conductance regulator and G-protein coupled receptors such as the beta2-adrenergic receptor and the parathyroid hormone 1 receptor. The protein also interacts with proteins that function as linkers between integral membrane and cytoskeletal proteins. The protein localizes to actin-rich structures including membrane ruffles, microvilli, and filopodia. Mutations in this gene result in hypophosphatemic nephrolithiasis/osteoporosis type 2, and loss of heterozygosity of this gene is implicated in breast cancer.
Images
 | Western blotting analysis using anti-NHERF Family PDZ Scaffold Protein 1 antibody (Cat#62529). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NHERF Family PDZ Scaffold Protein 1 antibody (Cat#62529, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-EBP50 antibody (Cat#62529). EBP50 expression in wild-type (WT) and NHERF1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-EBP50 antibody (Cat#62529, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of EBP50 expression in H9c2 cells using anti-EBP50 antibody (Cat#62529, 1:2,000). Green, isotype control; red, EBP50. |
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