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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | nemo like kinase |
| Gene Symbol: | NLK |
| Gene ID: | 51701 |
| UniProt ID: | Q9UBE8 |
| Clone ID: | 24GB585 |
| Immunogen: | A synthesized peptide derived from human NLK |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables DNA-binding transcription factor binding activity; protein serine/threonine kinase activity; and ubiquitin protein ligase binding activity. Involved in several processes, including negative regulation of TORC1 signaling; protein stabilization; and transforming growth factor beta receptor signaling pathway. Predicted to be located in cytosol and nucleoplasm. Predicted to be active in cytoplasm and nucleus.
Images
 | Western blotting analysis using anti-Nemo like kinase antibody (Cat#62384). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nemo like kinase antibody (Cat#62384, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Nemo like kinase antibody (Cat#62384). Nemo like kinase expression in wild type (WT) and Nemo like kinase shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Nemo like kinase antibody (Cat#62384, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Nemo like kinase expression in HeLa cells using Nemo like kinase antibody (Cat#62384, 1:2,000). Green, isotype control; red, Nemo like kinase. |
 | Immunocytochemical staining of HeLa cells with anti-Nemo like kinase antibody (Cat#62384, 1:1,000). Nuclei were stained blue with DAPI; Nemo like kinase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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