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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | nth like DNA glycosylase 1 |
| Gene Symbol: | NTHL1 |
| Synonyms: | FAP3; NTH1; OCTS3; hNTH1 |
| Gene ID: | 4913 |
| UniProt ID: | P78549 |
| Clone ID: | 25GB3020 |
| Immunogen: | A synthesized peptide derived from human NTH1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a DNA N-glycosylase of the endonuclease III family. Like a similar protein in E. coli, the encoded protein has DNA glycosylase activity on DNA substrates containing oxidized pyrimidine residues and has apurinic/apyrimidinic lyase activity.
Images
 | Western blotting analysis using anti-nth like DNA glycosylase 1 antibody (Cat#61611). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-nth like DNA glycosylase 1 antibody (Cat#61611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-nth like DNA glycosylase 1 antibody (Cat#61611). Nth like DNA glycosylase 1 expression in wild-type (WT) and nth like DNA glycosylase 1 (NTHL1) knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-nth like DNA glycosylase 1 antibody (Cat#61611, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Nth like DNA glycosylase 1 expression in HepG2 cells using anti-Nth like DNA glycosylase 1c antibody (Cat#61611, 1:2,000). Green, isotype control; red, Nth like DNA glycosylase 1. |
 | Immunocytochemical staining of Nth like DNA glycosylase 1 cells with Nth like DNA glycosylase 1antibody (Cat#61611, 1:1,000). Nuclei were stained blue with DAPI; Nth like DNA glycosylase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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