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| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phosphate cytidylyltransferase 1A, choline |
| Gene Symbol: | PCYT1A |
| Synonyms: | CT; CTA; CCTA; CGL5; CTPCT; PCYT1; SMDCRD; CCTalpha |
| Gene ID: | 5130 |
| UniProt ID: | P49585 |
| Clone ID: | 24GB5015 |
| Immunogen: | A synthesized peptide derived from human CTA |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the cytidylyltransferase family and is involved in the regulation of phosphatidylcholine biosynthesis. Mutations in this gene are associated with spondylometaphyseal dysplasia with cone-rod dystrophy. Alternatively spliced transcript variants have been found for this gene.
Images
 | Western blotting analysis using anti-PCYT1A antibody (Cat#63313). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCYT1A antibody (Cat#63313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PCYT1A antibody (Cat#63313). PCYT1A expression in wild-type (WT) and PCYT1A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PCYT1A antibody (Cat#63313, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PCYT1A expression in HepG2 cells using anti-PCYT1A antibody (Cat#63313, 1:2,000). Green, isotype control; red, PCYT1A. |
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