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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | PDZ and LIM domain 1 |
| Gene Symbol: | PDLIM1 |
| Synonyms: | CLIM1; CLP36; CLP-36; hCLIM1; HEL-S-112 |
| Gene ID: | 9124 |
| UniProt ID: | O00151 |
| Clone ID: | 24GB1310 |
| Immunogen: | A synthesized peptide derived from human PDLIM1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17.
Images
 | Western blotting analysis using anti-PDLIM1 antibody (Cat#62538). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDLIM1 antibody (Cat#62538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PDLIM1 antibody (Cat#62538). PDLIM1 expression in wild type (WT) and PDLIM1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-PDLIM1 antibody (Cat#62538, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PDLIM1 expression in HepG2 cells using anti-PDLIM1 antibody (Cat#62538, 1:2,000). Green, isotype control; red, PDLIM1. |
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