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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | 3-phosphoinositide dependent protein kinase 1 |
| Gene Symbol: | PDPK1 |
| Synonyms: | PDK1; 3-phosphoinositide-dependent protein kinase 1; hPDK1 |
| Gene ID: | 5170 |
| UniProt ID: | O15530 |
| Clone ID: | 24GB5425 |
| Immunogen: | A synthesized peptide derived from human PDPK1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables 3-phosphoinositide-dependent protein kinase activity; phospholipase activator activity; and phospholipase binding activity. Involved in several processes, including cell surface receptor signaling pathway; regulation of protein kinase activity; and regulation of signal transduction. Acts upstream of or within intracellular signal transduction. Located in cell projection; cytosol; and plasma membrane. Implicated in prostate cancer. Biomarker of lung non-small cell carcinoma.
Images
 | Western blotting analysis using anti-PDPK1 antibody (Cat#63385). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDPK1 antibody (Cat#63385, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). PDPK1, 3-phosphoinositide dependent protein kinase 1. |
 | Western blotting analysis using anti-PDPK1 antibody (Cat#63385). PDPK1 expression in wild type (WT) and PDPK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PDPK1 antibody (Cat#63385, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PDPK1 expression in HepG2 cells using anti-PDPK1 antibody (Cat#63385, 1:2,000). Green, isotype control; red, PDPK1. |
 | Immunocytochemical staining of HepG2 cells with anti-PDPK1 antibody (Cat#63385, 1:1,000). Nuclei were stained blue with DAPI; PDPK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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