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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phosphoglycerate kinase 1 |
| Gene Symbol: | PGK1 |
| Synonyms: | PGKA; MIG10; HEL-S-68p |
| Gene ID: | 5230 |
| UniProt ID: | P00558 |
| Clone ID: | 24GB2935 |
| Immunogen: | Recombinant protein of human PGK1. |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome.
Images
 | Western blotting analysis using anti-Phosphoglycerate kinase 1 antibody (Cat#62871). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phosphoglycerate kinase 1 antibody (Cat#62871, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-phosphoglycerate kinase 1 antibody (Cat#62871). Phosphoglycerate kinase 1 expression in wild-type (WT) and phosphoglycerate kinase 1 (PGK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phosphoglycerate kinase 1 antibody (Cat#62871, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Immunocytochemical staining of HepG2 cells with anti-Phosphoglycerate kinase 1 antibody (Cat#62871, 1:1,000). Nuclei were stained blue with DAPI; Phosphoglycerate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. |
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