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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | prohibitin 1 |
| Gene Symbol: | PHB1 |
| Synonyms: | PHB; BAP32; HEL-215; HEL-S-54e |
| Gene ID: | 5245 |
| UniProt ID: | P35232 |
| Clone ID: | 24GB6045 |
| Immunogen: | A synthesized peptide derived from human Prohibitin |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-prohibitin antibody (Cat#61170). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-prohibitin antibody (Cat#61170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-prohibitin antibody (Cat#61170). Prohibitin expression in wild type (WT) and prohibitin (PHB1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-prohibitin antibody (Cat#61170, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of prohibitin expression in HT-1080 cells using prohibitin antibody (Cat#61170, 1:2,000). Green, isotype control; red, prohibitin. |
 | Immunocytochemical staining of HT-1080 cells with prohibitin antibody (Cat#61170, 1:1,000). Nuclei were stained blue with DAPI; Prohibitin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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