Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | pyruvate kinase M1/2 |
| Gene Symbol: | PKM |
| Synonyms: | PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30 |
| Gene ID: | 5315 |
| UniProt ID: | P14618 |
| Clone ID: | 25GB2170 |
| Immunogen: | A synthesized peptide derived from human PKM2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
Images
 | Western blotting analysis using anti-PKM2 antibody (Cat#61305). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKM2 antibody (Cat#61305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PKM2 antibody (Cat#61305). PKM2 expression in wild-type (WT) and PKM knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PKM2 antibody (Cat#61305, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PKM2 expression in HT-1080 cells using PKM2 antibody (Cat#61305, 1:2,000). Green, isotype control; red, PKM2. |
 | Immunocytochemical staining of C2C12 cells with anti-PKM2 antibody (Cat#61305, 1:1,000). Nuclei were stained blue with DAPI; PKM2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
You may also be interested in:
