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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein phosphatase, Mg2+/Mn2+ dependent 1B |
| Gene Symbol: | PPM1B |
| Synonyms: | PP2CB; PP2CBETA; PP2C-beta; PPC2BETAX; PP2C-beta-X |
| Gene ID: | 5495 |
| UniProt ID: | O75688 |
| Clone ID: | 24GB13970 |
| Immunogen: | Recombinant protein of human PPM1B |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences.
Images
 | Western blotting analysis using anti-PPM1B antibody (Cat#65037). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PPM1B antibody (Cat#65037). PPM1B expression in wild-type (WT) and PPM1B shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PPM1B antibody (Cat#65037, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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