Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | protein kinase C alpha |
| Gene Symbol: | PRKCA |
| Synonyms: | AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha |
| Gene ID: | 5578 |
| UniProt ID: | P17252 |
| Clone ID: | 24GB4475 |
| Immunogen: | A synthesized peptide derived from human PKC alpha |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Images
 | Western blotting analysis using anti-PKC alpha antibody (Cat#63197). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC alpha antibody (Cat#63197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PKC alpha antibody (Cat#63197). PKC alpha expression in wild type (WT) and PRKCA shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PKC alpha antibody (Cat#63197, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PKC alpha expression in HepG2 cells using anti-PKC alpha antibody (Cat#63197, 1:2,000). Green, isotype control; red, PKC alpha. |
 | Immunocytochemical staining of HepG2 cells with anti-PKC alpha antibody (Cat#63197, 1:1,000). Nuclei were stained blue with DAPI; PKC alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
You may also be interested in:
