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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | protein kinase C alpha |
| Gene Symbol: | PRKCA |
| Synonyms: | AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha |
| Gene ID: | 5578 |
| UniProt ID: | P17252 |
| Immunogen: | A synthesized peptide derived from human PKC alpha |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Images
 | Western blotting analysis using anti-PRKCA antibody (Cat#65008). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRKCA antibody (Cat#65008, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-PRKCA antibody (Cat#65008). PRKCA expression in wild-type (WT) and PRKCA shRNA knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PRKCA antibody (Cat#65008, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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