KD-Validated PRKCD Rabbit mAb (20 μl)

KD-Validated PRKCD Rabbit mAb (20 μl)

Cat. #: 63200
Availability: In Stock
$149.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, FC    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Monoclonal antibody    
Gene Name:   protein kinase C delta
Gene Symbol:   PRKCD
Synonyms:   MAY1; PKCD; ALPS3; CVID9; nPKC-delta
Gene ID:   5580
UniProt ID:   Q05655
Clone ID:   24GB4485
Immunogen:   Recombinant protein of Mouse PKC delta (UniProt Entry: P28867)
Dilution:   WB 1:1,000-1:5,000; FC 1:2,000
Purification Method:   Affinity purified
Concentration:   Lot dependent
Buffer:   PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.




Background

The protein encoded by this gene is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrome.




Images

PRKCD antibodyWestern blotting analysis using anti-PKC delta antibody (Cat#63200). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PKC delta antibody (Cat#63200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PKC delta antibody (Cat#63200). PKC delta expression in wild-type (WT) and protein kinase C delta (PRKCD) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PKC delta antibody (Cat#63200, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of PKC delta expression in HAP-1 cells using anti-PKC delta antibody (Cat#63200, 1:2,000). Green, isotype control; red,PKC delta.



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