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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | PRKCSH beta subunit of glucosidase II |
| Gene Symbol: | PRKCSH |
| Synonyms: | GIIB; PCLD; PLD1; 80K-H; G19P1; PCLD1; PKCSH; AGE-R2; GIIbeta; VASAP-60; GluIIbeta |
| Gene ID: | 5589 |
| UniProt ID: | P14314 |
| Clone ID: | 24GB1325 |
| Immunogen: | A synthesized peptide derived from human GLU2B |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-PRKCSH antibody (Cat#62541). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRKCSH antibody (Cat#62541, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PRKCSH antibody (Cat#62541. PRKCSH expression in wild type (WT) and PRKCSH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PRKCSH antibody (Cat#62541, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of PRKCSH expression in Hela cells using anti-PRKCSH antibody (Cat#62541, 1:2,000). Green, isotype control; red, PRKCSH. |
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