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| Reactivity: | Human |
| Applications: | WB, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | cell division cycle 6 |
| Gene Symbol: | CDC6 |
| Synonyms: | CDC18L; HsCDC6; MGORS5; HsCDC18 |
| Gene ID: | 990 |
| UniProt ID: | Q99741 |
| Clone ID: | 24GB4770 |
| Immunogen: | A synthesized peptide derived from human Phospho-CDC6 (Ser106) |
| Dilution: | WB 1:1,000-1:5,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CDC6 encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Images
 | Western blotting analysis using anti-phospho-CDC6 (Ser106) antibody (Cat#63263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-phospho-CDC6 (Ser106) antibody (Cat#63263). Phospho-CDC6 (Ser106) expression in wild type (WT) and CDC6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-CDC6 (Ser106) antibody (Cat#63263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). CDC6, cell division cycle 6. |
 | Immunocytochemical staining of HepG2 cells with anti-phospho-CDC6 (Ser106) antibody (Cat#63263, 1:1,000). Nuclei were stained blue with DAPI; Phospho-CDC6 (Ser106) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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