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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | checkpoint kinase 1 |
| Gene Symbol: | CHEK1 |
| Synonyms: | CHK1; OZEMA21 |
| Gene ID: | 1111 |
| UniProt ID: | O14757 |
| Clone ID: | 25GB3390 |
| Immunogen: | A synthesized peptide derived from human Phospho-Chk1 (S280) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein CHEK1 encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Images
 | Western blotting analysis using anti-phospho-Chk1 (S280) antibody (Cat#61704). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-Chk1 (S280) antibody (Cat#61704, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-phospho-Chk1 (S280) antibody (Cat#61704). Phospho-Chk1 (S280) expression in wild-type (WT) and CHEK1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-Chk1 (S280) antibody (Cat#61704, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Phospho-Chk1 (S280) expression in HeLa cells using anti-Phospho-Chk1 (S280) antibody (Cat#61704, 1:2,000). Green, isotype control; red, Phospho-Chk1 (S280). |
 | Immunocytochemical staining of HeLa cells with anti-Phospho-Chk1 (S280) antibody (Cat#61704, 1:1,000). Nuclei were stained blue with DAPI; Phospho-Chk1 (S280) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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