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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | phospholipase C beta 3 |
| Gene Symbol: | PLCB3 |
| Synonyms: | SMDCD |
| Gene ID: | 5331 |
| UniProt ID: | Q01970 |
| Clone ID: | 24GB1260 |
| Immunogen: | A synthesized peptide derived from human Phospho-PLCB3 (S537) |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-Phospho-PLCB3 (S537) antibody (Cat#62526). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-PLCB3 (S537) antibody (Cat#62526, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-phospho-PLCB3 (S537) antibody (Cat#62526). Phospho-PLCB3 (S537) expression in wild-type (WT) and PLCB3 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-PLCB3 (S537) antibody (Cat#62526, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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